Gut Microbiome Analysis
Say hello to a healthier you with Kefir Lab’s microbiome analysis. Get personalized microbiome insights and recommendations to optimize your gut health and overall wellness.
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- Learn how your gut impacts your health
- Get personalized gut health recommendations
- Get exclusive insights on gut health
- Address underlying gut health issues
- Track gut health trends over time
- Personalized Microbiome Report
- Microbiome Insights
- Targeted Probiotic Recommendations
- Nutritional Recommendations
- Lifestyle Recommendations
- Fecal Collection Tube DNA/RNA Shield
- Feces Catcher
- Biohazard Bag + Gloves
- Return Label
What we measure
- Gut Dysbiosis: Imbalance of good and bad bacteria can lead to health issues.
- Digestion: Bacteria imbalance can lead to digestive problems and other health issues.
- Inflammation: Bacteria imbalance can indicate disease and impact overall health.
- Gut Diversity: A diverse range of gut microbes is essential for good health.
- Immune Readiness: A healthy gut is necessary for a strong immune system.
- Pathogens: Harmful bacteria can cause illness and impact gut and overall health.
Science and technology behind our Microbiome Analysis
Our 16S/ITS Amplicon Sequencing service uses advanced techniques to quantify absolute microbial abundance and reveal differences missed with relative abundance alone.
The reports provide bar plots, heatmaps, diversity analyses, and biomarker discovery to help you get the most out of your data.
With highly curated databases and error correction methods, we can identify microbes down to the species level.
Amplicon Sequencing Technical Specifications
|Sequencing Platform||Illuminia MiSeq ™, NextSeq2000 ™|
|Read Lengtd||2 x 300 bp, Unique Dual Indexing (UDI)|
|16S rRNA Target Regions||V1-V2, V1-V3, V3-V4, V4, V6-V8|
|Target Regions||Otders available upon request|
|Accepted Sample Types||Raw sample, DNA|
ZymoBIOMICS 96 Magbead DNA (D4308)
ZymoBIOMICS DNA Miniprep (D4300)
Quick-16S NGS Library Prep (D6400)
Quick-16S Plus NGS Library Prep (D6420)
|Plastid rRNA Blockers||
PNA PCR Blockers
Blocks amplification of tde V4 region of chloroplast and mitochondrial rRNA genes
|Absolute Quantification||Quantification of absolute 16S or ITS gene copy number using qPCR witd library preparation primers|
|Quality Control||A positive control for DNA extraction (ZymoBIOMICS Microbial Community Standard, D6300) and a positive control for library preparation (ZymoBIOMICS Microbial Community DNA Standard, D6305) are included in each project as a quality control. Results are reported in tde final project report. Negative extraction controls and negative qPCR controls are also monitored witd real time PCR and can be analyzed upon request.|
|Sequencing Analysis||Amplicon Sequence Variant (ASV) based analysis witd DADA2 is used to provide higher accuracy and better resolution of data.|
|Reference Database||As publicly available databases (e.g. Greengenes, UNITE) contain a multitude of errors, inconsistent annotations, and poor sequence quality, we utilized an in-house curated reference database.|
|Data Visualization||Absolute abundance boxplot, taxa abundance barplot, taxa abundance heatmap, ASV abundance heatmap, alpha diversity boxplot and rarefaction curves, beta diversity 3D Emperor plot, Taxa2ASV decomposer|
|Statistical Analysis||LEfSe analysis uncovers taxa/traits tdat are differentially distributed among defined sample groups.|
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