Sequencing Platform
Illumina NovaSeq 6000™, NextSeq 2000™, NovaSeq X™Sample Collection
Raw Sample
Sample collection kit is provided.
DNA sample
(> 10 ng/μl by Fluorescence DNA quantification)
Please ensure it is PCR inhibitor-free
Read Length
2 x 150 bp
Taxonomy Resolution
Bacteria and Archaea: strain-level
Fungi and Viruses: strain-level
Accepted Sample Types
Extracted Genomic DNA, or raw samplesDNA Extraction
ZymoBIOMICS 96 Magbead DNA (D4308)
ZymoBIOMICS DNA Miniprep (D4300)
Library Prep
Illumina DNA Prep (Formerly Nextera Flex)
Unique Dual Indexing (UDI) included
Sequencing Analysis
Kmer signature based analysis using Sourmash with antimicrobial resistance using Diamond and functional pathway analysis using Humann3 Reference Database
We use pre-built databases supported by the SourMash development group covering Prokaryote, Eukaryote, Archaea, and Viral genomes compatible with GTDB and NCBI taxonomies.
Data Visualization
Taxonomy Profiling
- Taxa abundance bar plot and heatmap
Functional Profiling:
- Antibiotics resistance identification
- Virulence gene identification
- Gene family profiling (UniRef)
- Metabolic pathway profiling (Metacyc)
Statistical Analysis
LEfSe analysis uncovers taxa/traits that are differentially distributed among defined sample groups.
Data Storage
Raw sequencing data and analysis report are stored for up to 3 months